|History | System specifications | Manuals/Docs ||
Leica provides a free, downloadable LCS Lite image editor capable of opening and editing Leica LCS program generated experiments.
At the beginning of 2004, the CSIF purchased, with a NIH Shared Instrument Grant (PI Minx Fuller) a Leica SP2 AOBS Confocal Laser Scanning Microscope.
The SP2 AOBS utilizes state of the art Acousto-Optical Beam Splitters (AOBS) in place of traditional glass di- or multichroics allowing user selection of reflecting excitation and transmitting emission wavelengths.
The confocal is equipped with a prism spectrometer detector which allows for spectral scanning of emission profiles as well as selection of emission detection bandwidth. This allows spectral detection and un-mixing of overlapping spectrum, e.g eCFP, eGFP, and eYFP expressed in the same cell.
These features make the new Leica confocal highly sensitive, flexible and, in addition to excelling in imaging fixed samples, is suited for live cell studies utilizing expressed FP-fusion proteins.
Microscope - DM IRE2 inverted microscope.
Software: Leica Confocal, v 2.5, build 1347
These computers run Leica software. Leica-PC is interfaced with the confocal and is for acquisition of images. St. Francis and Ashlar are the processing computers for analyzing data.
To obtain a copy of the CSIF user manual for this system, you will need to have Adobe Acrobat reader installed on your computer.