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Director
Jon Mulholland, 725-7532
e-mail:
jwm@stanford.edu
Jon Mulholland joined the Beckman Center's CSIF in 2001 and establised the new
Electron Microscopy Core while expanding existing fluorescent
microscopy resources. Prior to joining the CSIF he worked for many years in
the laboratory of Dr. David Botstein (starting at MIT, then Genetech, Inc. and finally Stanford). His research in the Botstein lab focused
on the ultrastructural biology of the yeast actin cytoskeleton and membrane vesicle
trafficking. While in the Botstein lab he successfully applied
immuno-EM methodologies which enabled the first immuno-EM study
of the actin cytoskeleton in yeast. He has expertise in both light
and electron microscopy. Reseach interests continue to be centered upon improved
light and electron imaging techniques for studying protein localization in yeast and other micro-
organisms.
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Fluorescence microscopy core staff:
Kitty Lee, 723-2449
e-mail:
csif@cmgm.stanford.edu
Kitty has been taking care of the CSIF confocal microscopes and their users
since 2002. She's a Hong Kong native who moved to the states in 1994.
As a double major in chem and bio with minors in education and
computer science, Kitty's college years were spent mostly outside her beloved
dorm room (split between cold computer room, creepy bio lab, nasty chem lab,
smelly animal facility, busy student health clinic etc, etc). She spent some
years at a neurobio lab that specializes in olfactory research, a
computational chem lab that seeks the least poisonous cancer-ridding chemical
conformation, a cognitive psych lab that tests human response to image
stimulus, and a PET scan research that compares human brain images (some of
which scored her authorship in a journal publication or two). Her addiction
to tantalizing CSIF users stems from her years of tutoring college students
and helping clinic nurses handling patients. Latest interest for Kitty
outside CSIF includes T.A.ing wilderness first aid class
(was once EMT-certified and enjoys playing with fake blood),
telemark skiing, snowcamping, kayaking and hiking/backpacking
(and shopping for all these activities). Kitty is an avid fencer who enjoys
fencing foil and playing with epees, please feel free to challenge her to a
duel after your confocal imaging sessions (she has a spare saber as well).
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Electron microscopy core staff:
John Perrino, 723-3462
e-mail:
jperrino@stanford.edu
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Lydia Joubert, 723-3462
e-mail:
lydiaj@stanford.edu
After majoring in Mathematics and Botany Lydia obtained her PhD in Plant Sciences, and did post-doctoral research on endophytic cyanobacteria-eukariotic plant symbiosis. Subsequent research on lignocellulolytic fungal enzymes led to an interest in degradative microbial consortia, and research in microbial ecology/environmental engineering, studying biofilms as complex microbial communities whose phenotypic plasticity affords the emergence of novel and superior competitive properties.
Visualization with Light and Electron Microscopy has been a central part of all her research and minimizing sample processing, as enabled by Variable Pressure SEM, became a focus point due to the hydrated nature and delicate 3D architecture of biofilms. Application of 'Wet SEM' techniques for nanotechnology application, cryofixation for SEM, and correlative light and electron microscopy are currently being developed and applied. She is also interestd in techniques for preserving and characterizing bacterial and yeast biofilms. Academic interests include earth systems theory, and application of games theory, as well as non-linearity, complexity and fractality to study biofilm behavior. Hobbies include lots of books and music, photography, jogging, skiing, hiking, piano-playing - and her family.
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